Composite

Part:BBa_K2557023

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-10)


EF1α promoter-PhiC31-RDF

According to the feedback of digital model, the recombination efficiency and expression level of recombinase-RDF will affect the stability of the switch we designed. Therefore, we need to characterize the promoters of different intensities and RDF, and hope to get an optimal combination scheme. The EF1α promoter and PhiC31-RDF are characterized here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 220
    Illegal BamHI site found at 1131
    Illegal BamHI site found at 1217
    Illegal BamHI site found at 1464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 298
    Illegal NgoMIV site found at 527
    Illegal NgoMIV site found at 569
    Illegal NgoMIV site found at 2140
    Illegal AgeI site found at 226
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 823
    Illegal BsaI.rc site found at 1226
    Illegal BsaI.rc site found at 1579
    Illegal BsaI.rc site found at 2221
    Illegal SapI.rc site found at 2823


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